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stemvision’s automated cfu assay reader and software  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc stemvision’s automated cfu assay reader and software
    Stemvision’s Automated Cfu Assay Reader And Software, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemvision%E2%80%99s+automated+cfu+assay+reader+and+software/stemvision+hematopoietic+colony+counter/pmc11176759__mmc2-239-7-11
    Average 90 stars, based on 1 article reviews
    stemvision’s automated cfu assay reader and software - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Colony-forming Unit Assay:

    Article Title: Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production
    Article Snippet: Twenty-four hours post transduction, cells were collected by centrifugation and suspended in fresh culture media to be either maintained in liquid culture (4 days for sort or 7 days for VCN), or to seed 1,800 cells in MethoCult H4434 Classic (Stemcell Technologies, Vancouver, Canda) for maintenance in STEMVision 6-well plates (Stemcell Technologies) at 37°C with CO 2 for 12 days. .. CFU-Cs were analyzed using STEMvision’s automated CFU assay reader and software (Stemcell Technologies), prior to being pooled for VCN analyses. .. Four days post transduction, 5 to 10 million CD34 + cells were stained with the following antibodies: hCD34-FITC (BioLegend, San Diego, CA; Cat #353504), hCD38-PE (BioLegend; Cat #356604), hCD90-APC (BioLegend; Cat #328114), hCD45RA-BV650 (BD Biosciences, San Jose, CA; Cat# 563963).

    Article Title: Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production
    Article Snippet: Twentyfour hours post transduction, cells were collected by centrifugation and suspended in fresh culture media to be either maintained in liquid culture (4 days for sort or 7 days for VCN), or to seed 1,800 cells in MethoCult H4434 Classic (Stemcell Technologies, Vancouver, Canda) for maintenance in STEMVision 6-well plates (Stemcell Technologies) at 37 C with CO2 for 12 days. .. CFU-Cs were analyzed using STEMvision’s automated CFU assay reader and software (Stemcell Technologies), prior to being pooled for VCN analyses. hCD34+ HSC staining and sorting Four days post transduction, 5 to 10 million CD34+ cells were stained with the following antibodies: hCD34-FITC (BioLegend, San Diego, CA; Cat #353504), hCD38-PE (BioLegend; Cat #356604), hCD90APC (BioLegend; Cat #328114), hCD45RA-BV650 (BD Biosciences, San Jose, CA; Cat# 563963). .. Live/dead cell marker was NucBlue Fixed Cell ReadyProbes reagent (DAPI) (ThermoFisher Scientific).

    Software:

    Article Title: Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production
    Article Snippet: Twenty-four hours post transduction, cells were collected by centrifugation and suspended in fresh culture media to be either maintained in liquid culture (4 days for sort or 7 days for VCN), or to seed 1,800 cells in MethoCult H4434 Classic (Stemcell Technologies, Vancouver, Canda) for maintenance in STEMVision 6-well plates (Stemcell Technologies) at 37°C with CO 2 for 12 days. .. CFU-Cs were analyzed using STEMvision’s automated CFU assay reader and software (Stemcell Technologies), prior to being pooled for VCN analyses. .. Four days post transduction, 5 to 10 million CD34 + cells were stained with the following antibodies: hCD34-FITC (BioLegend, San Diego, CA; Cat #353504), hCD38-PE (BioLegend; Cat #356604), hCD90-APC (BioLegend; Cat #328114), hCD45RA-BV650 (BD Biosciences, San Jose, CA; Cat# 563963).

    Article Title: Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production
    Article Snippet: Twentyfour hours post transduction, cells were collected by centrifugation and suspended in fresh culture media to be either maintained in liquid culture (4 days for sort or 7 days for VCN), or to seed 1,800 cells in MethoCult H4434 Classic (Stemcell Technologies, Vancouver, Canda) for maintenance in STEMVision 6-well plates (Stemcell Technologies) at 37 C with CO2 for 12 days. .. CFU-Cs were analyzed using STEMvision’s automated CFU assay reader and software (Stemcell Technologies), prior to being pooled for VCN analyses. hCD34+ HSC staining and sorting Four days post transduction, 5 to 10 million CD34+ cells were stained with the following antibodies: hCD34-FITC (BioLegend, San Diego, CA; Cat #353504), hCD38-PE (BioLegend; Cat #356604), hCD90APC (BioLegend; Cat #328114), hCD45RA-BV650 (BD Biosciences, San Jose, CA; Cat# 563963). .. Live/dead cell marker was NucBlue Fixed Cell ReadyProbes reagent (DAPI) (ThermoFisher Scientific).

    Staining:

    Article Title: Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production
    Article Snippet: Twentyfour hours post transduction, cells were collected by centrifugation and suspended in fresh culture media to be either maintained in liquid culture (4 days for sort or 7 days for VCN), or to seed 1,800 cells in MethoCult H4434 Classic (Stemcell Technologies, Vancouver, Canda) for maintenance in STEMVision 6-well plates (Stemcell Technologies) at 37 C with CO2 for 12 days. .. CFU-Cs were analyzed using STEMvision’s automated CFU assay reader and software (Stemcell Technologies), prior to being pooled for VCN analyses. hCD34+ HSC staining and sorting Four days post transduction, 5 to 10 million CD34+ cells were stained with the following antibodies: hCD34-FITC (BioLegend, San Diego, CA; Cat #353504), hCD38-PE (BioLegend; Cat #356604), hCD90APC (BioLegend; Cat #328114), hCD45RA-BV650 (BD Biosciences, San Jose, CA; Cat# 563963). .. Live/dead cell marker was NucBlue Fixed Cell ReadyProbes reagent (DAPI) (ThermoFisher Scientific).

    Transduction:

    Article Title: Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production
    Article Snippet: Twentyfour hours post transduction, cells were collected by centrifugation and suspended in fresh culture media to be either maintained in liquid culture (4 days for sort or 7 days for VCN), or to seed 1,800 cells in MethoCult H4434 Classic (Stemcell Technologies, Vancouver, Canda) for maintenance in STEMVision 6-well plates (Stemcell Technologies) at 37 C with CO2 for 12 days. .. CFU-Cs were analyzed using STEMvision’s automated CFU assay reader and software (Stemcell Technologies), prior to being pooled for VCN analyses. hCD34+ HSC staining and sorting Four days post transduction, 5 to 10 million CD34+ cells were stained with the following antibodies: hCD34-FITC (BioLegend, San Diego, CA; Cat #353504), hCD38-PE (BioLegend; Cat #356604), hCD90APC (BioLegend; Cat #328114), hCD45RA-BV650 (BD Biosciences, San Jose, CA; Cat# 563963). .. Live/dead cell marker was NucBlue Fixed Cell ReadyProbes reagent (DAPI) (ThermoFisher Scientific).



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    STEMCELL Technologies Inc stemvision’s automated cfu assay reader and software
    Stemvision’s Automated Cfu Assay Reader And Software, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemvision%E2%80%99s+automated+cfu+assay+reader+and+software/stemvision+hematopoietic+colony+counter/pmc11176759__mmc2-239-7-11
    Average 90 stars, based on 1 article reviews
    stemvision’s automated cfu assay reader and software - by Bioz Stars, 2026-09
    90/100 stars
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